Mec1-Dependent Phosphorylation of the Scc3 Subunit of Cohesin during Mitosis in Budding Yeast - Université de Tours Access content directly
Journal Articles Advances in Bioscience and Biotechnology Year : 2015

Mec1-Dependent Phosphorylation of the Scc3 Subunit of Cohesin during Mitosis in Budding Yeast

Abstract

Cohesin is an evolutionary conserved complex that controls chromosome segregation during mi-tosis. Here we show that, in response to DNA damage, Saccharomyces cerevisiae Scc3, one of the major regulatory subunits of the Smc1-Smc3-Scc1 cohesin ring, is phosphorylated on S/T-Q residues. This event depended on the Mec1 checkpoint kinase as well as on cell cycle arrest triggered by the DNA damage checkpoint network. This phosphorylation event also took place during mito-sis of an unperturbed cell cycle. The present finding that S. cerevisiae Scc3 is phosphorylated during mitosis represents a potentially important new regulatory step in cohesin's mitotic functions.
Fichier principal
Vignette du fichier
ABB_2015031715421497.pdf (3.64 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive
Loading...

Dates and versions

hal-01154292 , version 1 (21-05-2015)

Identifiers

Cite

Nathalie Grandin, Michel Charbonneau. Mec1-Dependent Phosphorylation of the Scc3 Subunit of Cohesin during Mitosis in Budding Yeast. Advances in Bioscience and Biotechnology, 2015, 06 (03), pp.153-163. ⟨10.4236/abb.2015.63015⟩. ⟨hal-01154292⟩

Collections

UNIV-TOURS CNRS
169 View
159 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More