Rvb2/reptin physically associates with telomerase in budding yeast
Abstract
Telomerase is a reverse transcriptase that maintains linear telomeres at a constant length. Here, we report that in the budding yeast Saccharomyces cerevisiae, Rvb2, a highly conserved member of the AAA+ family of ATPases, physically associates with telomerase/Est2 in vivo, both expressed from their endogenous promoter. Importantly, in genetic settings leading to a failure to recruit telome-rase at telomeric ends, Rvb2 still associated with Est2. On the other hand, Rvb2 was present in immunoprecipitates of crosslinked telomeric chromatin even in the presumed absence of telome-rase at the telomeres. Finally, we could also isolate RVB2 mutant alleles conferring slight, but stable, telomere shortening. Structured summary of protein interactions: Cdc13 physically interacts with Rvb2 by cross-linking study (View interaction) Est2 physically interacts with Rvb2 by anti tag coimmunoprecipitation (View interaction)
Domains
Molecular biology
Origin : Files produced by the author(s)
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