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Poster De Conférence Année : 2015

Innovative bioguided fractionation of cranberry juice for Candida biofilm treatment and infected-wound healing

Résumé

Cranberry polyphenols are intensively studied for their anti-infectious and antioxidant properties. Nevertheless, in biofilm and infected-wound healing, the identity of active polyphenols still remains unclear. In order to identify active polyphenols, an innovative fractionation scheme based on Centrifugal Partition Chromatography (CPC) was developed. CPC is a support free liquid/liquid chromatographic technology used here for the first time to fractionate cranberry juice. The fractionation protocol involved 3 mains steps: firstly polyphenol and sugars separation; then polyphenols fractionation based of molecular weight and polarity, and finally CPC or on LH-20 resin. Following the second step, the 6 main fractions obtained were screened on Candida biofilm and toward infected wound. Fractions containing low molecular weight proanthocyanidins (Fr Ill and IV) and containing high molecular weight PAC (Fr V ad VI) exhibited the most interesting activities. Concerning Candida albicans biofilm a prophylactic effect was observed by prevention of adhesion. On infected wound, a wound-healing potential related to anti-inflammatory effect but not to antibacterial activity against Staphylococcus aureus was highlighted. These four active fractions were further purified using either CPC or LH-20 resin, to lead to almost 40 sub-fractions. Against fractions containing PACs of medium and high molecular weight exhibited the highest activity against adhesion step of C. albicans strains and infected wound healing. This work is a step further to understand mechanisms of action of cranberry polyphenols on C. albicans biofilm prevention and in infected wound healing.
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Dates et versions

hal-01256486 , version 1 (03-06-2020)

Identifiants

  • HAL Id : hal-01256486 , version 1
  • PRODINRA : 342504

Citer

Leslie Boudesocque-Delaye, T. Puy, Marion Girardot, Arnaud Lanoue, Laurent Bazinet, et al.. Innovative bioguided fractionation of cranberry juice for Candida biofilm treatment and infected-wound healing. 7. International Conference on Polyphenols and Health, Oct 2015, Tours, France. , 2015. ⟨hal-01256486⟩
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