The role of GH receptor tyrosine phosphorylation in Stat5 activation - Université de Tours Access content directly
Journal Articles Journal of Molecular Endocrinology Year : 1997

The role of GH receptor tyrosine phosphorylation in Stat5 activation

J Hansen
  • Function : Author
L Hansen
  • Function : Author
J Kopchick
  • Function : Author
B Groner
  • Function : Author
J Nielsen
  • Function : Author
A Møldrup
  • Function : Author
E Galsgaard
  • Function : Author
N Billestrup
  • Function : Author
A Moldrup
  • Function : Author


Stimulation of GH receptors leads to rapid activation of Jak2 kinase and subsequent tyrosine phosphorylation of the GH receptor. Three specific tyrosines located in the C-terminal domain of the GH receptor have been identified as being involved in GH-stimulated transcription of the Spi 2.1 promoter. Mutated GH receptors lacking all but one of these three tyrosines are able to mediate a transcriptional response when transiently transfected into CHO cells together with a Spi 2.1 promoter/luciferase construct. Similarly, these GH receptors were found to be able to mediate activation of Stat5 DNA-binding activity, whereas the GH receptor mutant lacking all intracellular tyrosines was not. Synthetic tyrosine phosphorylated peptides corresponding to the GH receptor sequence around the three tyrosines inhibited Stat5 DNA-binding activity while their non-phosphorylated counterparts were ineffective. Tyrosine phosphorylated GST-GH receptor fusion proteins specifically bound to Stat5 in extracts from COS 7 cells transfected with Stat5 cDNA. This binding could be inhibited by tyrosine phosphorylated peptides derived from the GH receptor. This study thus demonstrated that specific GH receptor tyrosine residues, in their phosphorylated state, are involved in transcriptional signaling by directly interacting with Stat5.
Fichier principal
Vignette du fichier
The role of GH receptor tyrosine phosphorylation in.pdf (2.73 Mo) Télécharger le fichier
Origin : Publication funded by an institution

Dates and versions

hal-02427408 , version 1 (05-11-2021)



J Hansen, L Hansen, X. Wang, J Kopchick, F. Gouilleux, et al.. The role of GH receptor tyrosine phosphorylation in Stat5 activation. Journal of Molecular Endocrinology, 1997, 18 (3), pp.213-221. ⟨10.1677/jme.0.0180213⟩. ⟨hal-02427408⟩


12 View
46 Download



Gmail Facebook Twitter LinkedIn More