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Article Dans Une Revue Journal of Clinical Microbiology Année : 2018

Development and evaluation of a broad bead-based multiplex immunoassay to measure IgG seroreactivity against human polyomaviruses

Résumé

The family of polyomaviruses, which cause severe disease in immunocompromised hosts, has expanded substantially in recent years. To accommodate measurement of IgG seroresponses against all currently known human polyomaviruses (HPyVs), including the Lyon IARC polyomavirus (LIPyV), we extended our custom multiplex beadbased HPyV immunoassay and evaluated the performance of this pan-HPyV immunoassay. The VP1 proteins of 15 HPyVs belonging to 13 Polyomavirus species were expressed as recombinant glutathione S-transferase (GST) fusion proteins and coupled to fluorescent Luminex beads. Sera from healthy blood donors and immunocompromised kidney transplant recipients were used to analyze seroreactivity against the different HPyVs. For BK polyomavirus (BKPyV), the GST-VP1 fusion protein-directed seroresponses were compared to those obtained against BKPyV VP1 virus-like particles (VLP). Seroreactivity against most HPyVs was common and generally high in both test populations. Low seroreactivity against HPyV9, HPyV12, New Jersey PyV, and LIPyV was observed. The assay was reproducible (Pearson’s r2 > 0.84, P < 0.001) and specific. Weak but consistent cross-reactivity between the related viruses HPyV6 and HPyV7 was observed. The seroresponses measured by the GST-VP1-based immunoassay and a VP1 VLP-based enzymelinked immunosorbent assay were highly correlated (Spearman’s ρ = 0.823, P < 0.001). The bead-based pan-HPyV multiplex immunoassay is a reliable tool to determine HPyVspecific seroresponses with high reproducibility and specificity and is suitable for use in seroepidemiological studies.

Dates et versions

hal-02621616 , version 1 (26-05-2020)

Identifiants

Citer

Sergio Kamminga, Els van Der Meijden, Herman F. Wunderink, Antoine Touzé, Hans L. Zaaijer, et al.. Development and evaluation of a broad bead-based multiplex immunoassay to measure IgG seroreactivity against human polyomaviruses. Journal of Clinical Microbiology, 2018, 56 (4), pp.1-11. ⟨10.1128/JCM.01566-17⟩. ⟨hal-02621616⟩
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