Freezing Weakens the Barrier Function of Reconstructed Human Epidermis as Evidenced by Raman Spectroscopy and Percutaneous Permeation - Université de Tours Access content directly
Journal Articles Pharmaceutics Year : 2020

Freezing Weakens the Barrier Function of Reconstructed Human Epidermis as Evidenced by Raman Spectroscopy and Percutaneous Permeation

Yuri Dancik
Hichem Kichou
Martin Soucé
Emilie Munnier
Igor Chourpa

Abstract

The development and characterization of reconstructed human epidermis (RHE) is an active area of R&D. RHE can replace animal tissues in pharmaceutical, toxicological and cosmetic sciences, yielding scientific and ethical advantages. RHEs remain costly, however, due to consumables and time required for their culture and a short shelf-life. Storing, i.e., freezing RHE could help reduce costs but to date, little is known on the effects of freezing on the barrier function of RHE. We studied such effects using commercial EpiSkin™ RHE stored at −20, −80 and −150 °C for 1 and 10 weeks. We acquired intrinsic Raman spectra in the stratum corneum (SC) of the RHEs as well as spectra obtained following topical application of resorcinol in an aqueous solution. In parallel, we quantified the effects of freezing on the permeation kinetics of resorcinol from time-dependent permeation experiments. Principal component analyses discriminated the intrinsic SC spectra and the spectra of resorcinol-containing RHEs, in each case on the basis of the freezing conditions. Permeation of resorcinol through the frozen RHE increased 3- to 6-fold compared to fresh RHE, with the strongest effect obtained from freezing at −20 °C for 10 weeks. Due to the extensive optimization and standardization of EpiSkin™ RHE, the effects observed in our work may be expected to be more pronounced with other RHEs.

Dates and versions

hal-02989954 , version 1 (05-11-2020)

Identifiers

Cite

Yuri Dancik, Hichem Kichou, Christophe Eklouh-Molinier, Martin Soucé, Emilie Munnier, et al.. Freezing Weakens the Barrier Function of Reconstructed Human Epidermis as Evidenced by Raman Spectroscopy and Percutaneous Permeation. Pharmaceutics, 2020, 12 (11), pp.1041. ⟨10.3390/pharmaceutics12111041⟩. ⟨hal-02989954⟩

Collections

UNIV-TOURS
47 View
0 Download

Altmetric

Share

Gmail Facebook Twitter LinkedIn More