Raman spectral cytopathology for cancer diagnostic applications - Université de Tours Accéder directement au contenu
Article Dans Une Revue Nature Protocols Année : 2021

Raman spectral cytopathology for cancer diagnostic applications

Hugh Byrne

Résumé

Raman spectroscopy can provide a rapid, label-free, nondestructive measurement of the chemical fingerprint of a sample and has shown potential for cancer screening and diagnosis. Here we report a protocol for Raman microspectroscopic analysis of different exfoliative cytology samples (cervical, oral and lung), covering sample preparation, spectral acquisition, preprocessing and data analysis. The protocol takes 2 h 20 min for sample preparation, measurement and data preprocessing and up to 8 h for a complete analysis. A key feature of the protocol is that it uses the same sample preparation procedure as commonly used in diagnostic cytology laboratories (i.e., liquid-based cytology on glass slides), ensuring compatibility with clinical workflows. Our protocol also covers methods to correct for the spectral contribution of glass and sample pretreatment methods to remove contaminants (such as blood and mucus) that can obscure spectral features in the exfoliated cells and lead to variability. The protocol establishes a standardized clinical routine allowing the collection of highly reproducible data for Raman spectral cytopathology for cancer diagnostic applications for cervical and lung cancer and for monitoring suspicious lesions for oral cancer.
Fichier non déposé

Dates et versions

hal-03376450 , version 1 (13-10-2021)

Identifiants

Citer

Damien Traynor, Isha Behl, Declan O’dea, Franck Bonnier, Siobhan Nicholson, et al.. Raman spectral cytopathology for cancer diagnostic applications. Nature Protocols, 2021, 16 (7), pp.3716-3735. ⟨10.1038/s41596-021-00559-5⟩. ⟨hal-03376450⟩

Collections

UNIV-TOURS
19 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More