Optimization of Sample Preparation for Metabolomics Exploration of Urine, Feces, Blood and Saliva in Humans Using Combined NMR and UHPLC-HRMS Platforms - Université de Tours Accéder directement au contenu
Article Dans Une Revue (Data Paper) Molecules Année : 2021

Optimization of Sample Preparation for Metabolomics Exploration of Urine, Feces, Blood and Saliva in Humans Using Combined NMR and UHPLC-HRMS Platforms

Résumé

Currently, most clinical studies in metabolomics only consider a single type of sample such as urine, plasma, or feces and use a single analytical platform, either NMR or MS. Although some studies have already investigated metabolomics data from multiple fluids, the information is limited to a unique analytical platform. On the other hand, clinical studies investigating the human metabolome that combine multi-analytical platforms have focused on a single biofluid. Combining data from multiple sample types for one patient using a multimodal analytical approach (NMR and MS) should extend the metabolome coverage. Pre-analytical and analytical phases are time consuming. These steps need to be improved in order to move into clinical studies that deal with a large number of patient samples. Our study describes a standard operating procedure for biological specimens (urine, blood, saliva, and feces) using multiple platforms (1H-NMR, RP-UHPLC-MS, and HILIC-UHPLC-MS). Each sample type follows a unique sample preparation procedure for analysis on a multi-platform basis. Our method was evaluated for its robustness and was able to generate a representative metabolic map.
Fichier principal
Vignette du fichier
molecules-26-04111.pdf (1.31 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte
Licence : ETALAB - Licence Ouverte

Dates et versions

hal-03954853 , version 1 (24-01-2023)

Identifiants

Citer

Cécile Martias, Nadine Baroukh, Sylvie Mavel, Hélène Blasco, Antoine Lefèvre, et al.. Optimization of Sample Preparation for Metabolomics Exploration of Urine, Feces, Blood and Saliva in Humans Using Combined NMR and UHPLC-HRMS Platforms. Molecules, 2021, 26 (14), pp.4111. ⟨10.3390/molecules26144111⟩. ⟨hal-03954853⟩

Collections

UNIV-TOURS IBRAIN
8 Consultations
79 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More